Apex Hot Start Master Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 25ul of the 2.0X reaction mix.
For most standard applications Master Mix based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix with Buffer II is the best option.