PCRBIO Hot Start Taq DNA Polymerase from Genesee Scientific uses advanced antibody-mediated hot start technology for superior sensitivity. Whether you need a hot start assay for high throughput, automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
“hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer-dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small-molecule hot start technology offers improved specificity and sensitivity compared to other methods. PCRBIO Hot Start DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO Hot Start Taq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).