The GC5 Mix & Plate E. Coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. These cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium - Mix & Plate! The premade cells are highly efficient (> 10^8 transformants / μg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Supplied as a pack of 10 convenient 100 μl/tube single use aliquots or in a 96-tube format with removable 8-tube strips for your high-throughput transformation needs.
The Mix & Plate procedure will work for most transformations using Ampicillin selection and not requiring outgrowth. The highest transformation efficiencies can be obtained by incubating the cells with DNA on ice for 2-5 minutes (60 minutes maximum) prior to plating. When selecting with Kanamycin, Tetracycline, etc., an outgrowth performed in SOC medium is required for efficient transformation. In most cases, this step can be omitted when selecting with Ampicillin.