PCRBIO VeriFi Mix Red is a convenient high fidelity 2x mix designed for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates. The inclusion of a red dye enables direct loading and tracking during agarose gel electrophoresis.
PCRBIO VeriFi Mix Red contains the engineered and highly processive PCRBIO VeriFi Polymerase, developed for fast and versatile high fidelity PCR. The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.