The Mouse 5-hmC & 5-mC DNA Set is a set of organ specific mouse genomic DNAs isolated from a pool of 8-10 week old Swiss Webster mice. This genomic DNA set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified cytosines are present at physiologically relevant levels and loci. The levels of 5-mC and 5-hmC within the Mouse 5-hmC & 5-mC DNA Set have been precisely quantified by mass spectrometry (LC/MS) and been validated to be consistent by ELISA.