The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 μg (per prep) of high-quality RNA directly from samples in TRI-Reagent? or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent? to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (17 nucleotides or greater) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 5-10 minutes.
The RNA binding capacity of the supplied Zymo-Spin™ IIC Column is ~50 μg with a minimal elution volume of 25 μl.